Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 373.7 3
0055-0226
622.13 226.02 368 72.43 0.882
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 323.0 3
0056-0230
231.70 229.44 406 77.50 0.849
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 650.6 3
0057-0229
432.85 229.64 372 71.84 0.906
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 410.6 3
0058-0233
377.23 232.95 380 72.77 0.902
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 281.7 3
0059-0238
698.28 238.85 444 79.15 0.891
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 244.4 3
0060-0239
113.51 238.68 366 72.67 0.871
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 535.7 3
0061-0248
500.11 247.44 356 70.67 0.896
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 450.2 3
0062-0251
752.47 251.41 385 75.26 0.854
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 293.3 3
0063-0256
162.63 255.61 376 76.91 0.799
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 328.5 3
0064-0276
640.98 276.20 848 126.02 0.671
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 489.5 3
0065-0267
864.53 267.31 398 74.43 0.903
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2118.3 3
0066-0277
682.08 277.03 520 85.50 0.894
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 536.2 3
0067-0280
937.73 280.03 369 72.43 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 316.6 3
0068-0282
619.21 283.03 418 80.67 0.807
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 285.3 3
0069-0283
243.07 283.34 402 77.50 0.841
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 378.6 3
0070-0286
502.83 286.56 398 75.01 0.889
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1847.2 3
0071-0297
168.63 296.89 692 98.33 0.899
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 402.0 3
0072-0293
818.20 293.63 379 73.01 0.893
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 388.8 3
0073-0295
365.72 296.06 381 73.26 0.892
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 507.0 3
0074-0299
700.88 298.65 369 72.43 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 250.8 3
0075-0304
116.70 303.98 384 75.26 0.852
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 300.1 3
0076-0316
211.94 316.42 415 75.01 0.927
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 418.3 3
0077-0329
802.76 329.64 374 71.01 0.932
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 645.7 3
0078-0326
851.75 326.91 277 65.60 0.809
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 526.5 3
0079-0340
682.57 340.35 401 76.67 0.857