Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 411.8 1
0089-0341
 484.56 340.81 352 70.87 0.881
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 319.1 1
0090-0351
 904.71 350.58 428 78.08 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 311.5 1
0091-0352
 928.99 351.53 454 80.67 0.877
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 446.7 1
0092-0351
 95.71 351.12 406 75.84 0.887
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 405.7 1
0093-0357
 647.60 356.77 433 78.91 0.874
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 507.4 1
0094-0370
 457.83 370.44 943 123.64 0.775
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 520.7 1
0095-0361
 501.42 360.90 351 71.94 0.852
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 436.6 1
0096-0371
 663.63 371.45 456 84.57 0.801
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 299.5 1
0097-0368
 188.22 368.04 436 79.50 0.867
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 350.4 1
0098-0371
 596.97 371.40 448 79.84 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 261.3 1
0099-0370
 63.32 370.48 412 78.08 0.849
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 269.7 1
0100-0369
 275.88 369.77 423 76.43 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2334.6 1
0101-0379
 508.26 379.37 483 86.67 0.808
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 629.4 1
0102-0380
 816.50 380.84 437 79.50 0.869
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1301.4 1
0103-0392
 674.25 392.50 491 83.26 0.890
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 274.4 1
0104-0392
 134.34 392.60 425 76.43 0.914
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 452.8 1
0105-0395
 787.89 394.74 460 80.08 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1216.3 1
0106-0397
 710.62 397.69 428 77.84 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 365.7 1
0107-0404
 622.35 404.81 425 77.26 0.895
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2089.4 1
0108-0408
 927.12 408.71 622 92.91 0.905
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 614.2 1
0109-0408
 202.68 407.87 460 82.08 0.858
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 367.8 1
0110-0410
 447.45 410.02 447 79.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 427.9 1
0111-0410
 524.60 409.87 428 77.26 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 743.1 1
0112-0412
 230.55 412.57 450 80.08 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 506.8 1
0113-0421
 62.27 421.92 824 114.71 0.787