Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 151.6 8
0174-0525
 931.41 524.85 175 53.11 0.780
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 621.2 8
0175-0535
 746.35 535.25 891 126.47 0.700
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 456.0 8
0176-0527
 460.85 527.21 391 72.77 0.928
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 581.2 8
0177-0528
 366.17 528.27 407 78.67 0.826
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1354.1 8
0178-0533
 545.31 532.49 555 92.57 0.814
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 969.9 8
0179-0544
 226.15 544.19 422 78.08 0.870
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 273.5 8
0180-0546
 793.33 547.02 405 80.91 0.777
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 352.5 8
0181-0552
 696.30 551.94 410 78.67 0.833
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 358.3 8
0182-0560
 85.58 559.71 859 121.54 0.731
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 573.4 8
0183-0556
 404.04 556.74 407 75.50 0.897
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 533.9 8
0184-0562
 515.01 562.26 465 81.50 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 240.6 8
0185-0564
 800.48 565.21 445 86.08 0.755
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 325.2 8
0186-0569
 173.02 569.09 399 75.50 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 984.8 8
0187-0573
 556.41 572.86 403 75.60 0.886
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 280.8 8
0188-0578
 641.51 577.83 422 78.08 0.870
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 350.7 8
0189-0579
 740.58 578.80 406 75.50 0.895
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 427.0 8
0190-0580
 875.42 580.14 437 78.67 0.887
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 382.4 8
0191-0585
 44.22 584.44 406 84.81 0.709
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 769.3 8
0192-0581
 586.30 581.20 404 75.84 0.883
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 315.3 8
0193-0583
 324.64 583.53 438 79.50 0.871
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 311.7 8
0194-0589
 212.22 589.69 435 82.08 0.811
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 287.9 8
0195-0595
 263.91 595.35 398 77.50 0.833
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 485.6 8
0196-0598
 426.67 598.42 443 82.08 0.826
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1879.7 8
0197-0612
 39.00 612.44 432 80.57 0.836
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 257.7 8
0198-0611
 929.51 611.62 403 79.26 0.806