Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 773.9 6
0159-0450
 286.83 449.76 498 109.40 0.523
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 590.1 6
0160-0455
 546.95 455.76 440 81.26 0.837
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 384.8 6
0161-0473
 695.65 471.41 849 123.44 0.700
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 473.8 6
0162-0463
 767.08 463.73 453 79.94 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1930.3 6
0163-0470
 934.85 469.19 674 102.33 0.809
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 931.2 6
0164-0464
 910.91 463.51 355 70.77 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 351.6 6
0165-0469
 376.31 469.53 372 70.18 0.949
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 669.1 6
0166-0471
 177.58 471.75 413 78.67 0.839
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 460.2 6
0167-0472
 54.05 472.63 343 69.01 0.905
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 254.7 6
0168-0475
 592.81 474.91 415 75.26 0.921
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 364.6 6
0169-0480
 324.79 480.81 759 105.74 0.853
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 521.8 6
0170-0479
 435.04 479.50 376 72.18 0.907
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 793.0 6
0171-0483
 813.03 483.23 375 74.43 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 263.0 6
0172-0490
 770.88 489.39 662 102.91 0.785
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2314.9 6
0173-0486
 201.54 486.03 380 75.60 0.836
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 577.1 6
0174-0487
 278.85 487.89 358 69.84 0.922
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2478.1 6
0175-0490
 245.27 490.50 362 71.84 0.881
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 460.0 6
0176-0491
 477.77 491.04 428 78.08 0.882
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.6 6
0177-0498
 678.58 499.23 426 79.74 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 386.1 6
0178-0503
 118.99 502.66 441 82.57 0.813
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2366.4 6
0179-0501
 369.40 501.07 369 71.01 0.920
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 305.0 6
0180-0503
 572.50 503.11 376 74.08 0.861
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 250.4 6
0181-0507
 34.40 507.78 383 74.67 0.863
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1354.4 6
0182-0509
 934.67 508.54 372 73.01 0.877
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 504.3 6
0183-0515
 705.36 514.46 392 76.08 0.851