Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 885.6 6
0109-0311
 832.11 310.47 382 73.26 0.895
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 398.5 6
0110-0315
 475.27 315.03 395 74.18 0.902
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2973.5 6
0111-0319
 683.10 318.92 642 100.47 0.799
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 486.7 6
0112-0319
 301.13 319.45 382 73.84 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 867.3 6
0113-0327
 211.18 328.27 839 117.88 0.759
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 353.6 6
0114-0320
 566.22 320.72 385 75.50 0.849
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3235.8 6
0115-0320
 898.68 319.64 306 67.84 0.836
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 311.0 6
0116-0322
 354.69 321.99 394 75.50 0.869
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 798.0 6
0117-0322
 717.14 322.36 564 101.26 0.691
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 304.3 6
0118-0328
 640.59 328.33 362 75.26 0.803
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 445.1 6
0119-0334
 269.16 334.69 433 86.08 0.734
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 751.8 6
0120-0332
 495.27 331.52 399 74.18 0.911
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 968.5 6
0121-0337
 817.75 337.00 419 78.33 0.858
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3535.1 6
0122-0339
 909.62 339.16 468 81.74 0.880
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.7 6
0123-0340
 792.67 339.73 461 82.33 0.855
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 551.7 6
0124-0346
 23.77 346.14 733 109.64 0.766
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 307.0 6
0125-0347
 629.09 348.15 482 91.15 0.729
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 712.5 6
0126-0349
 772.87 349.59 347 73.74 0.802
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 602.2 6
0127-0350
 932.91 350.68 699 110.81 0.715
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.5 6
0128-0353
 876.46 352.96 385 73.01 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 365.2 6
0129-0358
 727.31 358.52 378 72.43 0.906
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 457.9 6
0130-0371
 408.14 370.44 795 118.02 0.717
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1618.9 6
0131-0366
 226.35 365.77 908 116.33 0.843
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 518.6 6
0132-0372
 118.56 372.22 342 68.77 0.909
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 553.3 6
0133-0380
 171.75 380.43 359 71.01 0.895