Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 455.8 9
0251-0520
 600.81 520.37 291 64.43 0.881
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 688.5 9
0252-0521
 190.24 520.79 236 61.94 0.773
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1115.7 9
0253-0526
 312.34 526.57 272 63.26 0.854
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4847.2 9
0254-0523
 382.37 523.68 259 60.87 0.878
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 357.6 9
0255-0532
 900.91 532.53 282 61.60 0.934
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 421.3 9
0256-0534
 743.07 534.31 283 63.60 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 518.2 9
0257-0536
 72.15 535.68 229 58.18 0.850
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1302.4 9
0258-0536
 345.34 536.25 264 60.53 0.906
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 957.2 9
0259-0541
 115.02 540.89 231 57.11 0.890
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4580.0 9
0260-0541
 388.16 540.96 250 62.04 0.816
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 870.6 9
0261-0544
 146.31 544.72 261 59.36 0.931
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4326.0 9
0262-0550
 208.62 549.77 372 71.01 0.927
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 413.2 9
0263-0549
 530.58 548.82 256 59.94 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 558.1 9
0264-0552
 65.45 553.53 305 69.74 0.788
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1073.9 9
0265-0551
 462.86 551.05 255 61.36 0.851
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 636.2 9
0266-0558
 187.19 558.95 291 65.11 0.863
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 601.4 9
0267-0560
 131.96 560.28 266 60.77 0.905
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1141.8 9
0268-0560
 300.81 559.95 236 57.36 0.902
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 316.9 9
0269-0562
 939.43 562.66 339 69.60 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 471.1 9
0270-0563
 49.26 563.13 288 64.18 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 537.5 9
0271-0564
 85.36 565.00 271 66.43 0.772
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2511.8 9
0272-0566
 339.62 565.46 310 65.36 0.912
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 536.6 9
0273-0569
 464.96 569.44 300 63.36 0.939
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 338.8 9
0274-0575
 736.81 575.78 288 64.77 0.863
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 522.7 9
0275-0577
 412.79 577.08 301 67.36 0.834