Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3641.6 6
0090-0191
672.91 190.78 309 70.18 0.788
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 522.7 6
0091-0193
940.32 193.32 259 59.70 0.913
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 830.6 6
0092-0198
822.87 198.48 341 74.67 0.769
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3259.5 6
0093-0203
625.45 203.38 295 65.84 0.855
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 967.3 6
0094-0202
909.82 202.56 250 58.77 0.910
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5887.3 6
0095-0201
807.76 202.00 102 63.01 0.323
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 530.6 6
0096-0206
129.34 206.23 298 64.18 0.909
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 406.2 6
0097-0209
397.85 209.34 257 61.60 0.851
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2766.2 6
0098-0211
432.42 211.02 231 61.11 0.777
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1063.4 6
0099-0210
500.04 211.04 243 59.36 0.867
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 436.4 6
0100-0210
568.93 209.67 290 63.60 0.901
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 340.5 6
0101-0215
215.75 215.14 308 65.01 0.916
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1381.2 6
0102-0218
279.31 219.19 265 61.46 0.882
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5006.0 6
0103-0222
723.31 222.66 271 62.53 0.871
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 419.5 6
0104-0225
618.20 224.48 300 63.60 0.932
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2363.3 6
0105-0229
887.94 229.13 329 68.18 0.889
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5405.3 6
0106-0231
59.71 230.72 334 66.77 0.941
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 327.5 6
0107-0236
91.16 236.34 285 63.26 0.895
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3340.4 6
0108-0237
773.38 237.52 324 67.36 0.897
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1219.2 6
0109-0236
279.03 236.04 389 74.18 0.888
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5018.7 6
0110-0236
669.75 236.45 282 62.43 0.909
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 407.8 6
0111-0238
343.16 238.63 321 67.84 0.876
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 493.0 6
0112-0242
573.88 243.29 297 69.84 0.765
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 442.9 6
0113-0244
756.21 244.60 290 66.18 0.832
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 567.4 6
0114-0240
940.51 240.31 252 60.77 0.858