Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4472.7 4
0106-0245
 716.17 245.07 436 77.60 0.910
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3057.8 4
0107-0247
 327.17 247.13 466 82.43 0.862
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1056.4 4
0108-0246
 134.48 245.89 176 49.36 0.908
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 499.4 4
0109-0253
 433.59 253.15 267 60.77 0.909
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1878.8 4
0110-0253
 587.73 252.99 305 65.01 0.907
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 379.7 4
0111-0256
 80.26 256.04 257 61.36 0.858
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5363.1 4
0112-0257
 247.39 255.62 261 61.60 0.864
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2345.7 4
0113-0256
 364.30 256.01 292 63.94 0.897
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1365.0 4
0114-0254
 936.70 254.54 298 65.36 0.877
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 626.3 4
0115-0257
 755.30 256.76 273 62.43 0.880
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 650.6 4
0116-0257
 864.11 257.95 306 66.77 0.863
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4165.6 4
0117-0258
 384.05 257.99 249 58.28 0.921
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2561.6 4
0118-0260
 777.97 259.88 263 59.94 0.920
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 351.5 4
0119-0265
 223.78 265.45 272 63.94 0.836
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1340.6 4
0120-0264
 502.47 264.27 314 66.43 0.894
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3224.2 4
0121-0265
 114.70 264.56 274 62.77 0.874
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 745.8 4
0122-0271
 280.71 271.29 271 61.36 0.905
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 421.2 4
0123-0271
 834.78 271.50 274 61.01 0.925
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1053.9 4
0124-0274
 894.80 274.10 305 65.60 0.891
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3226.6 4
0125-0278
 522.30 279.48 304 69.01 0.802
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 507.8 4
0126-0276
 602.65 276.21 294 62.77 0.938
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3093.4 4
0127-0284
 128.97 286.07 617 101.40 0.754
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5031.5 4
0128-0277
 254.08 276.78 243 59.36 0.867
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 336.0 4
0129-0286
 42.86 285.69 296 64.43 0.896
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 395.4 4
0130-0286
 158.65 286.05 218 55.70 0.883