Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3145.0 4
0056-0141
832.51 140.76 395 73.01 0.931
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 678.3 4
0057-0141
698.12 140.93 279 61.94 0.914
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 485.4 4
0058-0144
401.87 144.67 298 65.94 0.861
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2209.0 4
0059-0141
608.51 141.51 293 63.11 0.924
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 5266.4 4
0060-0143
890.55 143.94 350 69.01 0.923
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 345.3 4
0061-0147
281.89 147.32 272 62.53 0.874
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 910.6 4
0062-0155
211.91 154.96 244 59.11 0.877
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 423.0 4
0063-0155
546.55 155.53 283 63.70 0.876
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1627.2 4
0064-0157
650.36 156.76 250 58.18 0.928
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1479.6 4
0065-0162
471.64 161.79 460 83.98 0.820
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4166.4 4
0066-0162
875.55 162.57 335 67.01 0.937
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 512.1 4
0067-0165
392.06 164.70 252 60.87 0.855
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 670.9 4
0068-0162
908.00 162.89 262 59.11 0.942
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 387.4 4
0069-0166
15.51 165.91 257 61.01 0.868
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2041.6 4
0070-0165
626.78 165.37 265 61.36 0.885
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 415.8 4
0071-0169
55.25 169.81 268 63.94 0.824
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 397.2 4
0072-0173
930.06 173.44 334 73.60 0.775
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 468.3 4
0073-0171
572.45 171.06 277 65.01 0.824
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 353.2 4
0074-0175
117.62 175.02 347 70.18 0.885
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3069.0 4
0075-0174
608.69 174.77 343 72.67 0.816
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2281.3 4
0076-0176
709.60 175.55 246 58.77 0.895
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 962.7 4
0077-0182
743.35 181.60 249 57.94 0.932
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 274.8 4
0078-0187
260.34 187.52 305 68.43 0.819
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2533.3 4
0079-0187
396.30 186.68 288 65.01 0.856
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 577.5 4
0080-0189
524.11 189.77 271 62.53 0.871