Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2656.8 8
0372-0603
 122.93 603.29 228 56.77 0.889
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2922.7 8
0373-0602
 745.82 602.88 254 59.70 0.896
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3515.8 8
0374-0608
 772.61 607.70 396 82.08 0.739
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 823.3 8
0375-0611
 588.28 610.98 281 61.11 0.945
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1570.7 8
0376-0612
 691.92 612.85 280 65.36 0.824
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1610.4 8
0377-0611
 621.03 610.64 266 65.11 0.788
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1038.1 8
0378-0615
 660.18 615.09 219 54.77 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3343.9 8
0379-0615
 91.00 615.30 225 55.11 0.931
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 706.7 8
0380-0622
 353.66 621.48 274 67.60 0.754
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2053.0 8
0381-0617
 484.70 617.51 235 57.46 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2426.7 8
0382-0619
 283.71 620.14 193 51.46 0.916
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2701.4 8
0383-0624
 540.61 624.20 286 64.43 0.866
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2692.3 8
0384-0623
 809.43 623.21 223 55.60 0.907
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3356.0 8
0385-0626
 369.46 626.27 240 59.11 0.863
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2948.7 8
0386-0628
 750.05 628.17 455 80.08 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1912.5 8
0387-0626
 315.90 625.91 218 54.77 0.913
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1347.7 8
0388-0630
 400.01 630.46 315 65.01 0.937
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2716.9 8
0389-0634
 103.08 633.11 250 61.60 0.828
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 7335.3 8
0390-0633
 301.76 632.85 182 52.77 0.821
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 340.8 8
0391-0634
 920.99 635.17 270 65.01 0.803
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2729.5 8
0392-0641
 826.35 642.57 525 91.01 0.796
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 862.6 8
0393-0636
 250.17 635.64 243 57.11 0.936
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 9657.2 8
0394-0636
 286.29 635.91 299 67.11 0.834
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2176.2 8
0395-0640
 56.08 640.78 309 64.18 0.943
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3867.5 8
0396-0645
 176.16 644.35 531 89.74 0.829