Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2738.3 8
0022-0056
 493.18 56.48 338 69.01 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 950.8 8
0023-0054
 940.44 54.25 205 52.28 0.942
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1740.2 8
0024-0061
 241.09 61.83 193 56.77 0.753
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 10071.7 8
0025-0056
 768.83 55.64 147 45.80 0.881
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1089.0 8
0026-0060
 866.08 60.35 250 59.36 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1228.9 8
0027-0063
 559.18 62.41 294 67.60 0.809
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 462.2 8
0028-0063
 642.81 63.02 178 48.28 0.959
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3219.1 8
0029-0064
 701.82 64.72 262 60.77 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1237.7 8
0030-0068
 91.77 68.89 240 59.11 0.863
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2011.8 8
0031-0070
 346.19 69.91 273 61.94 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2663.1 8
0032-0069
 838.43 68.84 215 56.18 0.856
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 8813.5 8
0033-0070
 765.62 69.56 157 46.77 0.902
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 974.7 8
0034-0071
 878.70 71.36 219 55.70 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1210.5 8
0035-0075
 248.18 76.27 218 55.36 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2102.6 8
0036-0076
 753.87 77.01 208 55.01 0.864
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2255.1 8
0037-0078
 789.59 77.65 266 62.18 0.864
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5190.1 8
0038-0080
 204.05 79.65 217 54.53 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 569.1 8
0039-0082
 535.30 81.33 202 55.46 0.825
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3155.6 8
0040-0086
 684.70 86.65 407 78.67 0.826
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2489.3 8
0041-0081
 911.11 81.40 246 57.70 0.929
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3861.0 8
0042-0083
 278.01 82.10 183 51.36 0.872
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3446.9 8
0043-0083
 422.56 82.95 209 52.87 0.940
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3539.0 8
0044-0092
 521.47 91.87 335 73.11 0.788
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1105.0 8
0045-0087
 654.93 87.18 272 62.77 0.868
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2190.9 8
0046-0088
 803.61 88.22 262 60.77 0.892