Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1103.4 5
0180-0350
 388.40 350.64 213 55.70 0.863
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3783.4 5
0181-0355
 313.02 354.74 290 68.67 0.773
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1148.6 5
0182-0363
 367.52 363.21 367 73.74 0.848
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2903.3 5
0183-0361
 868.51 361.52 228 55.70 0.924
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3699.2 5
0184-0364
 481.68 364.53 243 59.36 0.867
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2210.6 5
0185-0366
 744.53 366.18 361 73.84 0.832
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2136.1 5
0186-0370
 155.81 369.80 243 59.36 0.867
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1656.0 5
0187-0368
 583.87 367.91 293 63.01 0.927
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1144.6 5
0188-0369
 793.88 369.56 267 62.18 0.868
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 554.5 5
0189-0374
 425.48 374.39 298 65.36 0.877
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 998.3 5
0190-0376
 815.09 375.59 267 71.01 0.665
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 7701.2 5
0191-0377
 708.52 377.08 346 67.60 0.952
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1235.0 5
0192-0379
 228.04 379.25 321 67.60 0.883
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5924.9 5
0193-0382
 762.06 381.50 344 70.18 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2385.4 5
0194-0384
 331.51 384.50 264 59.94 0.923
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1769.4 5
0195-0383
 57.91 383.42 245 60.18 0.850
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 674.3 5
0196-0384
 638.43 383.82 173 55.36 0.709
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2424.7 5
0197-0385
 22.61 385.34 298 64.77 0.893
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1471.5 5
0198-0387
 437.65 386.44 260 61.70 0.858
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1171.8 5
0199-0387
 264.16 387.60 220 54.87 0.918
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 308.0 5
0200-0388
 941.49 389.46 247 73.26 0.578
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1099.6 5
0201-0387
 298.35 387.32 267 60.77 0.909
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3637.8 5
0202-0388
 545.48 388.10 243 57.70 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1578.3 5
0203-0390
 123.06 389.49 212 55.36 0.869
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5343.9 5
0204-0390
 474.10 389.95 175 51.36 0.834