Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 638.7 5
0105-0212
 363.48 212.07 256 61.94 0.838
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 944.2 5
0106-0207
 608.13 207.61 120 42.28 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 7945.8 5
0107-0213
 243.40 213.91 322 69.26 0.844
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1875.4 5
0108-0216
 625.16 216.59 319 66.18 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3815.7 5
0109-0223
 312.51 224.14 373 80.33 0.726
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 683.4 5
0110-0221
 154.93 221.42 238 57.70 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 711.2 5
0111-0222
 388.83 221.77 269 61.60 0.891
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 753.7 5
0112-0224
 462.43 223.48 257 59.94 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4277.5 5
0113-0227
 527.41 227.24 242 57.46 0.921
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1003.1 5
0114-0231
 138.45 230.97 229 55.94 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1517.9 5
0115-0231
 354.39 231.96 211 54.53 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3416.9 5
0116-0235
 717.67 234.91 391 75.01 0.873
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5089.6 5
0117-0232
 327.87 231.96 209 53.70 0.911
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 692.1 5
0118-0233
 485.95 233.10 274 61.36 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4530.1 5
0119-0234
 549.68 234.97 201 53.36 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 486.6 5
0120-0236
 920.16 237.33 301 67.84 0.822
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3955.0 5
0121-0238
 698.47 238.19 231 56.53 0.908
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1373.1 5
0122-0243
 786.65 243.09 305 70.43 0.773
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1175.8 5
0123-0243
 182.01 242.88 185 52.53 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2132.9 5
0124-0247
 806.30 247.73 346 69.01 0.913
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4539.6 5
0125-0248
 342.03 248.20 243 61.60 0.805
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2980.9 5
0126-0246
 747.93 245.72 211 53.70 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1115.3 5
0127-0251
 627.61 251.69 278 63.70 0.861
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1305.9 5
0128-0251
 839.54 251.41 302 67.01 0.845
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1009.7 5
0129-0253
 672.20 253.35 290 66.67 0.820