Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1030.9 4
0183-0386
576.32 386.15 240 59.84 0.842
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3742.4 4
0184-0387
418.73 387.11 311 64.18 0.949
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 6165.9 4
0185-0390
124.30 390.25 186 53.36 0.821
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 389.1 4
0186-0394
365.20 394.49 281 66.67 0.794
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1219.3 4
0187-0391
494.81 391.49 250 62.18 0.812
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 489.7 4
0188-0398
183.49 398.08 301 66.18 0.864
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1004.4 4
0189-0400
98.22 399.80 254 77.84 0.527
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1805.0 4
0190-0400
135.37 401.02 223 54.77 0.934
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1787.9 4
0191-0405
156.03 405.04 308 66.43 0.877
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 737.7 4
0192-0408
821.50 408.19 249 62.77 0.794
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4005.6 4
0193-0411
614.01 410.08 287 65.01 0.853
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 6891.4 4
0194-0410
649.13 410.07 302 67.01 0.845
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 8468.9 4
0195-0417
395.36 417.62 367 72.67 0.873
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 890.6 4
0196-0415
479.20 415.09 240 58.53 0.880
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1947.0 4
0197-0416
556.77 415.96 302 66.43 0.860
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1713.3 4
0198-0417
942.83 416.70 256 59.11 0.921
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 513.1 4
0199-0419
913.28 418.81 232 57.94 0.868
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2221.6 4
0200-0427
43.01 427.15 337 67.36 0.933
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1895.9 4
0201-0426
245.01 425.35 253 63.36 0.792
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 6171.7 4
0202-0432
322.69 431.90 417 78.08 0.859
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 6256.1 4
0203-0430
351.01 431.03 219 58.53 0.803
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2490.9 4
0204-0437
619.88 438.32 364 82.43 0.673
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2537.2 4
0205-0437
93.59 437.36 451 82.08 0.841
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1929.0 4
0206-0436
698.93 436.19 371 71.84 0.903
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3381.0 4
0207-0440
172.17 439.91 341 68.18 0.922