Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 209.7 8
0003-0061
 843.75 61.43 216 66.04 0.622
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 230.1 8
0004-0072
 744.01 72.77 201 59.70 0.709
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1476.0 8
0005-0110
 290.92 109.83 264 63.01 0.836
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 298.4 8
0006-0124
 868.05 124.44 204 55.36 0.837
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.0 8
0007-0136
 397.97 136.22 279 62.53 0.897
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 324.9 8
0008-0157
 297.33 157.19 199 51.11 0.957
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.6 8
0009-0177
 236.55 176.67 260 64.53 0.785
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 315.3 8
0010-0177
 353.95 177.55 204 52.28 0.938
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 236.8 8
0011-0182
 141.63 182.29 215 60.43 0.740
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 290.3 8
0012-0184
 417.53 184.50 266 63.84 0.820
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 326.1 8
0013-0195
 580.65 195.20 189 53.11 0.842
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.9 8
0014-0207
 918.96 206.97 220 55.60 0.894
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 263.3 8
0015-0214
 236.87 214.53 211 59.01 0.761
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 271.8 8
0016-0241
 897.47 241.14 199 55.36 0.816
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 270.2 8
0017-0260
 527.98 260.45 217 56.18 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 273.3 8
0018-0264
 450.21 263.65 272 63.36 0.852
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 241.7 8
0019-0297
 861.92 297.03 274 63.94 0.842
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.3 8
0020-0298
 714.96 298.45 291 64.18 0.888
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 239.9 8
0021-0330
 699.62 330.32 173 47.46 0.965
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 256.5 8
0022-0334
 721.44 334.50 230 69.26 0.603
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.0 8
0023-0343
 708.13 343.65 123 45.11 0.759
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.1 8
0024-0356
 95.62 357.04 246 59.36 0.877
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 205.3 8
0025-0358
 770.82 358.20 230 63.60 0.715
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 283.3 8
0026-0376
 353.60 378.03 264 77.26 0.556
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 262.5 8
0027-0378
 259.72 378.03 213 55.36 0.874