Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 210.4 9
0087-0457
 627.33 457.38 328 68.18 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 260.3 9
0088-0460
 250.17 459.58 271 62.18 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 327.2 9
0089-0460
 360.06 459.62 272 61.36 0.908
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 192.7 9
0090-0466
 762.09 465.46 350 71.01 0.872
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 225.3 9
0091-0473
 583.58 473.73 297 64.18 0.906
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 284.6 9
0092-0483
 354.63 482.60 295 64.18 0.900
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.1 9
0093-0490
 838.00 490.06 300 64.77 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 210.2 9
0094-0496
 656.41 496.27 342 72.53 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 223.3 9
0095-0495
 418.64 495.14 284 62.77 0.906
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 189.7 9
0096-0498
 792.53 497.76 331 69.36 0.865
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 187.1 9
0097-0496
 774.17 495.84 350 71.94 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.7 9
0098-0499
 52.74 498.30 283 62.43 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 218.3 9
0099-0501
 727.06 500.76 271 61.94 0.888
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.2 9
0100-0505
 207.71 504.91 249 58.18 0.924
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 215.4 9
0101-0521
 78.62 521.30 261 65.01 0.776
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 225.2 9
0102-0521
 614.23 521.50 371 70.18 0.946
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 242.3 9
0103-0525
 681.54 525.40 290 65.36 0.853
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.3 9
0104-0524
 710.85 524.45 293 63.11 0.924
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 253.1 9
0105-0536
 359.76 536.61 335 68.43 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 212.6 9
0106-0539
 624.88 539.71 358 72.08 0.866
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 238.9 9
0107-0545
 414.30 545.05 278 68.08 0.754
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 235.7 9
0108-0543
 740.94 544.00 305 65.94 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 211.6 9
0109-0551
 127.64 551.73 323 70.67 0.813
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.6 9
0110-0555
 376.56 555.19 274 60.77 0.932
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 222.7 9
0111-0559
 311.67 559.17 348 69.60 0.903