Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 212.3 8
0047-0254
 91.96 254.52 319 73.70 0.738
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 341.8 8
0048-0259
 345.30 258.91 262 60.77 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 186.6 8
0049-0267
 175.50 267.03 342 69.60 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 239.1 8
0050-0268
 413.70 268.21 323 71.50 0.794
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 226.6 8
0051-0269
 476.36 269.60 262 62.87 0.833
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 248.2 8
0052-0267
 608.28 266.91 333 69.01 0.879
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 543.7 8
0053-0276
 824.72 276.05 241 71.26 0.596
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 309.3 8
0054-0280
 327.52 280.38 308 69.94 0.791
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 226.7 8
0055-0286
 908.01 285.70 313 68.53 0.838
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.8 8
0056-0287
 257.30 287.08 289 64.18 0.882
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 199.7 8
0057-0296
 114.24 295.98 271 60.28 0.937
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 230.9 8
0058-0301
 527.71 301.18 325 70.43 0.823
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 305.8 8
0059-0303
 773.32 302.44 372 73.26 0.871
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 249.5 8
0060-0301
 67.03 300.89 265 60.77 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 202.2 8
0061-0306
 726.59 306.20 334 67.60 0.919
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 221.8 8
0062-0307
 454.46 307.65 280 64.77 0.839
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 213.3 8
0063-0312
 484.66 310.84 355 71.01 0.885
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.8 8
0064-0309
 802.16 310.13 312 67.01 0.873
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 270.1 8
0065-0309
 596.25 308.86 334 68.77 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 239.2 8
0066-0316
 693.21 316.50 327 69.94 0.840
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 406.1 8
0067-0314
 860.26 314.34 217 55.94 0.871
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 244.2 8
0068-0321
 66.27 321.41 288 64.18 0.879
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 208.2 8
0069-0340
 518.16 340.62 344 70.18 0.878
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 218.9 8
0070-0341
 265.73 342.16 331 68.18 0.895
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 224.5 8
0071-0351
 434.28 351.13 305 66.53 0.866