Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 405.4 2
0026-0156
 215.70 157.45 411 85.50 0.707
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.5 2
0027-0157
 165.99 157.66 306 66.77 0.863
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 266.8 2
0028-0162
 411.80 162.46 265 61.36 0.885
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 318.5 2
0029-0166
 749.08 166.72 255 59.36 0.910
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 924.1 2
0030-0170
 119.40 169.22 339 71.01 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 361.3 2
0031-0169
 139.86 169.16 344 68.53 0.921
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 231.0 2
0032-0176
 510.70 176.42 377 72.43 0.903
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 281.6 2
0033-0172
 635.63 171.65 154 45.94 0.917
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 249.3 2
0034-0178
 457.29 178.32 334 68.77 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 286.7 2
0035-0181
 626.08 181.30 201 55.01 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 280.8 2
0036-0187
 645.35 186.92 276 63.60 0.857
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 353.2 2
0037-0189
 845.17 189.53 216 59.60 0.764
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 233.6 2
0038-0193
 325.10 193.61 304 64.53 0.917
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 229.4 2
0039-0198
 409.45 197.88 339 70.18 0.865
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 234.8 2
0040-0209
 535.17 208.71 386 73.26 0.904
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 227.6 2
0041-0222
 15.25 222.86 325 68.53 0.870
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 227.8 2
0042-0224
 616.40 224.46 300 65.01 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 317.2 2
0043-0226
 843.78 226.33 273 63.60 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 246.5 2
0044-0230
 121.18 229.88 332 68.18 0.897
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.1 2
0045-0228
 239.63 228.40 268 65.01 0.797
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 205.2 2
0046-0230
 652.00 230.29 317 67.84 0.866
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 241.8 2
0047-0233
 715.12 232.41 190 55.11 0.786
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 306.9 2
0048-0235
 323.12 235.58 304 71.11 0.755
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 258.0 2
0049-0245
 404.92 245.37 349 71.84 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 247.8 2
0050-0250
 276.28 249.47 274 63.36 0.858