Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 212.4 4
0153-0518
459.30 518.98 448 80.08 0.878
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 192.8 4
0154-0525
620.07 525.23 571 92.33 0.842
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 254.5 4
0155-0527
836.57 527.33 282 64.18 0.860
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 992.6 4
0156-0529
360.05 529.94 429 79.36 0.856
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 387.8 4
0157-0536
213.40 535.85 972 125.30 0.778
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 237.9 4
0158-0534
407.41 533.57 504 85.74 0.862
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 431.0 4
0159-0535
579.10 535.16 529 91.40 0.796
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 343.7 4
0160-0534
23.69 534.42 407 75.26 0.903
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 251.7 4
0161-0535
718.74 534.88 466 81.26 0.887
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 1193.4 4
0162-0550
73.80 550.34 433 80.08 0.848
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 275.6 4
0163-0551
781.30 551.45 459 79.01 0.924
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 1910.8 4
0164-0552
361.37 551.17 526 89.60 0.823
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 251.1 4
0165-0554
444.12 555.08 426 78.67 0.865
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 190.7 4
0166-0560
496.05 559.90 449 79.26 0.898
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 245.8 4
0167-0561
717.51 561.75 514 86.33 0.867
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 295.4 4
0168-0562
409.60 562.68 472 82.33 0.875
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 187.0 4
0169-0567
933.70 566.31 553 96.13 0.752
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 216.7 4
0170-0565
628.41 565.59 465 80.67 0.898
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 197.1 4
0171-0568
32.00 567.88 411 75.01 0.918
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 258.3 4
0172-0573
259.57 571.93 451 81.40 0.855
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 217.5 4
0173-0574
546.75 574.82 461 81.26 0.877
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 258.5 4
0174-0577
109.77 577.72 423 75.26 0.939
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 326.4 4
0175-0581
316.05 581.14 554 88.08 0.897
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 331.1 4
0176-0584
909.84 584.85 803 114.95 0.764
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-2
S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
S6 (pS235; pS236) 203.1 4
0177-0579
803.61 578.83 480 81.50 0.908