COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 255.8 | 3 | 0094-0198 | 831.79 | 197.32 | 370 | 75.60 | 0.814 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 2223.0 | 3 | 0095-0197 | 49.38 | 196.72 | 237 | 58.18 | 0.880 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 452.5 | 3 | 0096-0197 | 513.77 | 197.29 | 315 | 65.01 | 0.937 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 302.6 | 3 | 0097-0204 | 457.17 | 203.08 | 271 | 61.01 | 0.915 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 497.4 | 3 | 0098-0203 | 704.26 | 203.91 | 332 | 73.01 | 0.783 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 280.4 | 3 | 0099-0204 | 354.49 | 204.48 | 347 | 73.01 | 0.818 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 1037.5 | 3 | 0100-0208 | 569.50 | 207.85 | 443 | 78.67 | 0.900 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 297.0 | 3 | 0101-0210 | 948.56 | 209.54 | 264 | 63.11 | 0.833 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 295.8 | 3 | 0102-0214 | 469.57 | 213.88 | 255 | 59.94 | 0.892 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 344.9 | 3 | 0103-0225 | 924.52 | 225.67 | 367 | 87.15 | 0.607 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 271.7 | 3 | 0104-0228 | 644.11 | 227.88 | 315 | 70.77 | 0.790 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 290.5 | 3 | 0105-0225 | 61.98 | 224.71 | 299 | 70.67 | 0.752 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 249.0 | 3 | 0106-0225 | 160.87 | 224.84 | 322 | 69.84 | 0.830 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 481.2 | 3 | 0107-0229 | 862.41 | 228.41 | 317 | 68.67 | 0.845 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 266.9 | 3 | 0108-0228 | 18.02 | 227.83 | 235 | 55.94 | 0.944 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 341.2 | 3 | 0109-0234 | 751.08 | 233.32 | 289 | 64.18 | 0.882 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 914.0 | 3 | 0110-0236 | 782.55 | 236.23 | 246 | 57.36 | 0.940 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 227.0 | 3 | 0111-0240 | 93.05 | 240.20 | 308 | 66.77 | 0.868 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 318.4 | 3 | 0112-0238 | 892.29 | 239.00 | 287 | 65.01 | 0.853 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 617.0 | 3 | 0113-0240 | 235.55 | 240.11 | 332 | 67.36 | 0.920 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 283.9 | 3 | 0114-0237 | 585.30 | 237.86 | 152 | 46.87 | 0.869 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 422.7 | 3 | 0115-0241 | 269.32 | 240.96 | 307 | 66.77 | 0.865 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 455.8 | 3 | 0116-0248 | 610.00 | 249.03 | 258 | 62.28 | 0.836 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 695.4 | 3 | 0117-0246 | 373.04 | 246.03 | 343 | 76.43 | 0.738 |
COLO 858 | Vemurafenib | 0.0316 | uM | 48 | hr | | | Cycle-2 | S6 (pS235; pS236) / Alexa Fluor 647 | rabbit IgG(H+L) / goat / cross-adsorbed | Cy5 | S6 (pS235; pS236) | | 529.0 | 3 | 0118-0248 | 765.67 | 248.01 | 239 | 67.01 | 0.669 |