Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 457.4 1
0351-0623
 362.48 623.47 330 71.36 0.814
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 297.1 1
0352-0630
 892.17 629.37 254 61.01 0.857
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 333.0 1
0353-0630
 162.83 629.67 272 62.53 0.874
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 411.2 1
0354-0629
 806.37 629.13 187 53.70 0.815
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 12953.9 1
0355-0635
 848.75 635.77 360 72.18 0.868
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 261.3 1
0356-0634
 489.75 634.91 317 71.84 0.772
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 309.7 1
0357-0634
 93.78 634.92 259 59.60 0.916
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 428.2 1
0358-0636
 243.32 636.51 374 72.67 0.890
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 405.3 1
0359-0638
 562.17 638.24 302 63.94 0.928
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 277.6 1
0360-0642
 508.02 640.75 239 59.60 0.846
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 280.7 1
0361-0644
 63.05 643.58 112 40.28 0.867
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 341.6 1
0362-0644
 693.93 644.85 228 56.87 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.3 1
0363-0647
 734.79 647.28 278 62.77 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 300.7 1
0364-0650
 783.99 651.00 192 58.53 0.704
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 300.4 1
0365-0650
 313.67 650.57 254 60.77 0.864
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.1 1
0366-0649
 401.23 648.55 262 61.11 0.882
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 544.0 1
0367-0653
 199.09 653.50 315 65.60 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 305.8 1
0368-0653
 447.88 652.57 345 68.77 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.9 1
0369-0651
 74.63 651.70 142 47.11 0.804
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 590.7 1
0370-0663
 237.20 663.32 593 97.98 0.776
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 285.2 1
0371-0654
 359.27 653.72 266 61.01 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 308.8 1
0372-0659
 46.25 659.41 273 61.94 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 415.7 1
0373-0661
 180.10 661.22 361 70.43 0.915
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.2 1
0374-0661
 327.81 660.85 284 63.01 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.5 1
0375-0662
 546.66 662.74 419 79.01 0.843