Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1304.1 3
0304-0506
 271.95 507.29 292 65.36 0.859
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 4943.3 3
0305-0506
 197.87 506.15 325 67.94 0.885
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 5053.7 3
0306-0504
 326.24 504.30 201 54.53 0.850
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2721.3 3
0307-0506
 749.41 506.57 218 54.87 0.910
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3383.3 3
0308-0512
 233.99 511.86 404 77.84 0.838
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 3829.5 3
0309-0508
 309.33 507.70 254 59.36 0.906
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2941.8 3
0310-0512
 409.95 512.07 328 68.18 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1869.8 3
0311-0512
 920.21 511.04 368 74.43 0.835
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 632.4 3
0312-0511
 945.71 510.79 299 69.01 0.789
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1779.3 3
0313-0513
 162.91 512.93 234 55.70 0.948
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 503.2 3
0314-0519
 641.88 518.69 471 82.08 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1298.9 3
0315-0518
 433.87 518.13 300 65.60 0.876
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1417.9 3
0316-0515
 807.92 515.37 204 53.94 0.881
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2145.9 3
0317-0516
 879.29 515.82 275 62.77 0.877
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1975.3 3
0318-0520
 468.34 519.83 387 74.18 0.884
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1475.1 3
0319-0525
 92.32 525.27 242 61.94 0.793
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1879.5 3
0320-0528
 892.42 527.57 242 59.94 0.846
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2335.7 3
0321-0534
 563.20 534.52 290 64.43 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1259.1 3
0322-0538
 930.82 537.99 358 70.43 0.907
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 973.3 3
0323-0544
 338.86 544.18 447 80.08 0.876
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2280.5 3
0324-0543
 615.93 543.94 313 68.87 0.829
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 1790.1 3
0325-0545
 291.44 545.26 385 74.91 0.862
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 4562.9 3
0326-0543
 687.91 543.10 258 62.43 0.832
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2270.8 3
0327-0542
 802.99 542.92 224 57.94 0.838
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS235; pS236) / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 S6 (pS235; pS236) 2194.1 3
0328-0547
 65.80 547.79 283 70.53 0.715