Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 327.9 8
0037-0478
 172.46 478.59 190 52.77 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 672.2 8
0038-0478
 451.26 478.26 172 50.18 0.858
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 296.4 8
0039-0485
 204.35 484.39 245 61.60 0.811
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 230.9 8
0040-0493
 109.12 493.18 278 65.94 0.803
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 200.1 8
0041-0514
 550.29 513.57 307 69.94 0.789
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1534.1 8
0042-0513
 707.35 513.93 288 62.18 0.936
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 212.8 8
0043-0518
 868.54 518.43 202 61.01 0.682
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1583.8 8
0044-0518
 406.70 519.39 142 45.21 0.873
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 311.1 8
0045-0536
 565.66 536.59 212 64.04 0.650
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1519.6 8
0046-0533
 407.92 532.20 168 48.63 0.893
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 860.4 8
0047-0534
 301.31 534.72 230 59.11 0.827
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2562.0 8
0048-0548
 192.92 547.90 260 61.36 0.868
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1069.0 8
0049-0556
 511.92 556.09 302 66.18 0.866
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 314.1 8
0050-0566
 299.97 566.75 193 53.60 0.844
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 236.2 8
0051-0580
 924.69 579.23 294 65.36 0.865
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 577.8 8
0052-0589
 270.62 588.33 268 63.11 0.845
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 230.6 8
0053-0595
 880.07 594.80 355 73.36 0.829
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1141.0 8
0054-0610
 218.90 609.47 260 67.84 0.710
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 9762.5 8
0055-0621
 146.69 622.02 198 57.11 0.763
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 343.2 8
0056-0621
 290.70 621.09 249 60.18 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1161.3 8
0057-0639
 577.61 640.06 225 62.53 0.723
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 317.8 8
0058-0657
 135.73 657.10 210 61.70 0.693
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 497.7 8
0059-0652
 674.40 652.07 277 69.36 0.724
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1865.7 8
0060-0666
 415.93 666.00 171 55.11 0.707
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1387.4 8
0061-0699
 370.97 699.10 183 51.70 0.860