Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2358.5 4
0011-0184
 270.60 185.09 156 47.46 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2324.8 4
0012-0184
 258.83 184.32 117 42.63 0.809
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2587.2 4
0013-0198
 252.47 198.14 271 61.60 0.898
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2583.7 4
0014-0202
 271.16 202.46 228 62.77 0.727
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 317.8 4
0015-0222
 683.17 222.21 211 63.60 0.656
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 263.7 4
0016-0223
 626.05 223.64 268 61.60 0.888
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 359.4 4
0017-0230
 296.16 230.32 363 80.67 0.701
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 755.1 4
0018-0236
 870.80 235.72 251 60.53 0.861
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 429.5 4
0019-0248
 297.88 248.35 171 67.84 0.467
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 469.7 4
0020-0251
 61.22 251.90 222 54.77 0.930
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 194.8 4
0021-0252
 929.07 252.31 258 60.77 0.878
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 246.3 4
0022-0261
 757.81 261.64 281 65.01 0.835
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1442.0 4
0023-0260
 858.20 260.13 218 61.70 0.720
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1271.5 4
0024-0273
 154.52 273.23 239 64.53 0.721
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 306.3 4
0025-0274
 848.11 274.54 288 65.94 0.832
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 311.1 4
0026-0278
 57.57 278.85 196 52.53 0.893
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 225.6 4
0027-0290
 821.95 289.82 232 58.53 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 662.6 4
0028-0297
 30.71 296.88 302 66.77 0.851
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 367.4 4
0029-0300
 383.48 300.35 174 49.11 0.907
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 276.6 4
0030-0308
 258.64 308.85 209 57.60 0.792
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 798.2 4
0031-0310
 101.43 310.42 257 62.53 0.826
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 267.6 4
0032-0312
 542.78 311.58 301 68.77 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 266.9 4
0033-0325
 554.36 324.38 243 67.01 0.680
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 371.1 4
0034-0343
 635.95 342.84 195 59.01 0.704
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 530.1 4
0035-0351
 370.80 351.24 187 53.70 0.815