Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1000.0 8
0031-0192
 128.31 192.14 319 68.77 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3422.6 8
0032-0198
 834.68 198.26 273 61.36 0.911
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 778.8 8
0033-0199
 509.73 199.88 314 73.60 0.728
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1185.6 8
0034-0200
 934.39 200.06 339 68.77 0.901
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 310.0 8
0035-0203
 451.50 203.05 309 66.77 0.871
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 716.3 8
0036-0212
 809.27 211.52 260 59.94 0.909
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 201.5 8
0037-0212
 298.17 211.31 305 67.36 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 308.6 8
0038-0219
 171.70 218.50 349 70.18 0.890
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 227.0 8
0039-0233
 637.45 233.56 392 80.67 0.757
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1195.9 8
0040-0231
 389.44 231.39 302 67.36 0.837
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 221.0 8
0041-0237
 69.26 237.61 245 60.43 0.843
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 212.0 8
0042-0246
 216.23 246.26 271 64.43 0.820
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2007.4 8
0043-0252
 368.24 252.48 258 60.18 0.895
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 603.8 8
0044-0255
 555.78 254.76 308 67.01 0.862
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 6825.8 8
0045-0255
 786.48 254.80 372 71.60 0.912
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1340.7 8
0046-0255
 269.76 254.60 284 66.43 0.809
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 200.7 8
0047-0254
 91.96 254.52 319 73.70 0.738
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2172.9 8
0048-0259
 345.30 258.91 262 60.77 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 203.3 8
0049-0267
 175.50 267.03 342 69.60 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 741.2 8
0050-0268
 413.70 268.21 323 71.50 0.794
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1674.5 8
0051-0269
 476.36 269.60 262 62.87 0.833
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 239.8 8
0052-0267
 608.28 266.91 333 69.01 0.879
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 270.6 8
0053-0276
 824.72 276.05 241 71.26 0.596
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 363.0 8
0054-0280
 327.52 280.38 308 69.94 0.791
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 221.0 8
0055-0286
 908.01 285.70 313 68.53 0.838