Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 962.9 5
0149-0649
392.44 648.81 314 67.60 0.864
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 266.4 5
0150-0651
477.69 651.41 393 74.43 0.892
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2298.0 5
0151-0651
648.86 650.78 265 63.26 0.832
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 456.1 5
0152-0656
839.71 656.03 262 65.60 0.765
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 254.6 5
0153-0652
916.58 652.30 326 69.94 0.837
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 306.4 5
0154-0660
61.87 660.43 318 67.84 0.868
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 268.8 5
0155-0669
717.31 668.92 367 74.33 0.835
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1734.8 5
0156-0675
309.34 674.77 300 67.01 0.840
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1201.8 5
0157-0674
400.17 675.66 347 73.84 0.800
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 708.9 5
0158-0679
451.47 679.38 325 69.84 0.837
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 343.3 5
0159-0684
636.39 684.58 320 66.18 0.918
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 206.7 5
0160-0696
906.66 696.33 348 73.01 0.820
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3856.0 6
0001-0040
323.34 40.63 347 73.01 0.818
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1656.7 6
0002-0047
782.00 47.24 273 67.26 0.758
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 680.4 6
0003-0053
426.76 53.37 270 66.67 0.763
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2197.5 6
0004-0055
149.57 55.43 286 63.94 0.879
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1164.1 6
0005-0060
25.83 60.11 304 64.77 0.911
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 624.8 6
0006-0061
823.37 60.79 253 65.84 0.733
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 917.9 6
0007-0067
247.36 66.94 265 65.60 0.774
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 269.7 6
0008-0077
673.88 77.38 377 71.84 0.918
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2226.0 6
0009-0084
318.86 83.55 340 71.26 0.842
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 625.4 6
0010-0082
527.49 83.00 305 64.77 0.914
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 817.7 6
0011-0087
691.40 87.25 305 68.43 0.819
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 513.2 6
0012-0093
196.33 91.42 299 70.18 0.763
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3201.7 6
0013-0093
411.70 93.66 250 59.60 0.884