Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 228.7 5
0099-0411
271.43 411.55 382 72.77 0.907
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 325.6 5
0100-0411
806.69 411.17 317 66.18 0.909
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 660.5 5
0101-0410
931.79 410.06 268 63.01 0.848
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 211.3 5
0102-0416
163.49 417.53 505 84.33 0.892
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 296.6 5
0103-0412
488.23 411.26 280 63.60 0.870
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 399.5 5
0104-0415
603.65 415.15 316 68.18 0.854
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 323.0 5
0105-0423
356.28 423.04 348 72.43 0.834
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 259.1 5
0106-0427
40.59 427.14 247 64.53 0.745
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1451.6 5
0107-0430
435.55 429.89 458 82.33 0.849
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 240.2 5
0108-0440
211.05 440.67 269 62.77 0.858
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 287.4 5
0109-0439
640.00 439.36 249 57.94 0.932
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 363.8 5
0110-0442
417.16 442.94 262 61.01 0.884
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 222.1 5
0111-0443
899.55 443.27 301 64.18 0.918
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1471.9 5
0112-0447
476.08 447.55 363 70.67 0.913
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 231.7 5
0113-0451
734.64 451.15 297 66.18 0.852
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 278.2 5
0114-0456
242.30 455.90 332 69.60 0.861
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 221.7 5
0115-0451
578.34 451.24 303 68.77 0.805
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1795.3 5
0116-0469
58.94 469.69 252 61.60 0.835
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 271.6 5
0117-0471
351.25 471.21 327 70.43 0.828
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 273.4 5
0118-0472
551.99 471.88 329 67.11 0.918
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 622.4 5
0119-0472
203.72 471.30 258 61.94 0.845
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 285.0 5
0120-0478
503.26 478.79 262 67.11 0.731
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 222.5 5
0121-0485
856.17 484.58 358 83.15 0.651
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 228.1 5
0122-0485
677.69 485.26 282 61.36 0.941
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1002.0 5
0123-0487
399.87 488.02 359 73.50 0.835