Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 795.6 4
0074-0466
 560.47 466.09 336 71.01 0.837
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 259.9 4
0075-0477
 753.31 477.02 509 85.50 0.875
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2368.7 4
0076-0487
 519.08 487.44 347 70.43 0.879
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 269.6 4
0077-0489
 901.11 489.35 224 59.70 0.790
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 210.5 4
0078-0497
 148.51 496.66 310 65.60 0.905
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 697.4 4
0079-0504
 288.85 504.09 327 67.26 0.908
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 747.1 4
0080-0502
 21.26 502.29 265 65.60 0.774
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 502.0 4
0081-0502
 230.11 502.30 295 68.43 0.792
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 258.7 4
0082-0507
 702.74 507.36 250 59.94 0.874
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 444.3 4
0083-0518
 434.28 517.40 308 65.60 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1814.3 4
0084-0517
 327.21 518.02 295 65.60 0.861
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 279.6 4
0085-0532
 654.71 531.73 370 71.36 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 636.3 4
0086-0535
 143.11 534.87 285 64.18 0.869
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 769.9 4
0087-0536
 197.18 535.93 334 68.18 0.903
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 888.7 4
0088-0538
 519.41 538.85 295 65.01 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2654.2 4
0089-0547
 598.89 547.62 236 57.11 0.909
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 281.2 4
0090-0553
 770.22 549.80 273 100.71 0.338
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1210.6 4
0091-0553
 308.70 552.52 334 68.43 0.896
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 201.0 4
0092-0554
 236.15 553.92 325 72.18 0.784
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 936.9 4
0093-0553
 374.13 553.74 227 60.43 0.781
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 309.3 4
0094-0561
 844.19 562.12 348 72.67 0.828
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 275.1 4
0095-0561
 791.09 560.82 264 83.50 0.476
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 229.6 4
0096-0566
 270.03 566.14 283 65.84 0.820
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 142.3 4
0097-0565
 19.85 565.60 260 60.53 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1544.4 4
0098-0581
 691.35 581.50 300 65.36 0.883