Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 274.9 2
0035-0181
 626.08 181.30 201 55.01 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 263.2 2
0036-0187
 645.35 186.92 276 63.60 0.857
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 487.1 2
0037-0189
 845.17 189.53 216 59.60 0.764
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 724.2 2
0038-0193
 325.10 193.61 304 64.53 0.917
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 649.6 2
0039-0198
 409.45 197.88 339 70.18 0.865
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 479.5 2
0040-0209
 535.17 208.71 386 73.26 0.904
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 208.6 2
0041-0222
 15.25 222.86 325 68.53 0.870
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 424.3 2
0042-0224
 616.40 224.46 300 65.01 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 269.2 2
0043-0226
 843.78 226.33 273 63.60 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 217.2 2
0044-0230
 121.18 229.88 332 68.18 0.897
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 375.0 2
0045-0228
 239.63 228.40 268 65.01 0.797
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 664.1 2
0046-0230
 652.00 230.29 317 67.84 0.866
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 6871.2 2
0047-0233
 715.12 232.41 190 55.11 0.786
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 352.2 2
0048-0235
 323.12 235.58 304 71.11 0.755
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 330.8 2
0049-0245
 404.92 245.37 349 71.84 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 635.1 2
0050-0250
 276.28 249.47 274 63.36 0.858
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 225.2 2
0051-0264
 929.92 263.21 249 63.01 0.788
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 991.5 2
0052-0261
 191.74 262.17 309 69.36 0.807
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2596.4 2
0053-0261
 360.66 261.16 226 56.04 0.904
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 536.7 2
0054-0268
 54.40 268.60 344 69.60 0.892
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 262.0 2
0055-0269
 887.42 269.70 221 56.87 0.859
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1117.7 2
0056-0270
 341.91 269.66 282 63.94 0.867
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 681.2 2
0057-0270
 377.36 269.33 302 67.01 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 403.7 2
0058-0280
 724.41 279.94 346 72.18 0.834
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 432.0 2
0059-0287
 837.58 288.01 278 63.36 0.870