Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 653.4 6
0027-0102
 836.77 101.45 590 115.05 0.560
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 236.8 6
0028-0099
 255.89 99.19 382 75.50 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2833.3 6
0029-0100
 595.90 99.78 365 72.67 0.869
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 227.7 6
0030-0109
 422.07 109.20 401 74.67 0.904
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1580.0 6
0031-0126
 484.81 126.11 881 126.71 0.690
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 675.6 6
0032-0131
 39.36 130.44 645 96.08 0.878
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 897.5 6
0033-0131
 549.78 131.58 381 73.84 0.878
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1536.4 6
0034-0131
 623.75 131.32 378 73.01 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 275.6 6
0035-0132
 172.95 132.44 376 77.26 0.792
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 460.1 6
0036-0138
 663.74 138.16 402 76.08 0.873
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 274.9 6
0037-0140
 893.42 140.11 365 71.60 0.895
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 409.2 6
0038-0141
 808.06 140.48 388 75.84 0.848
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 206.2 6
0039-0144
 271.47 144.83 389 76.57 0.834
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2802.1 6
0040-0146
 420.14 146.29 720 105.98 0.806
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 261.0 6
0041-0145
 457.69 145.41 336 70.18 0.857
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 903.3 6
0042-0145
 731.89 144.61 357 71.84 0.869
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 371.1 6
0043-0147
 106.08 147.62 398 74.67 0.897
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1123.6 6
0044-0146
 132.63 146.74 389 80.57 0.753
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 334.0 6
0045-0148
 301.61 148.60 373 73.50 0.868
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 605.4 6
0046-0148
 505.48 148.16 399 77.01 0.845
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 276.1 6
0047-0149
 838.37 149.10 369 72.67 0.878
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1064.7 6
0048-0153
 190.42 152.90 356 80.43 0.692
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1140.3 6
0049-0159
 767.90 159.50 381 75.01 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 901.8 6
0050-0159
 605.24 159.53 411 76.67 0.879
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 326.5 6
0051-0163
 326.95 162.93 371 72.43 0.889