Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 959.1 3
0013-0058
 54.92 58.96 377 80.43 0.732
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 255.4 3
0014-0064
 74.21 63.65 384 77.26 0.809
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 369.8 3
0015-0063
 927.38 63.53 352 71.26 0.871
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 530.8 3
0016-0065
 350.14 65.53 386 73.84 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 817.0 3
0017-0069
 250.38 69.79 363 77.26 0.764
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3845.1 3
0018-0067
 904.78 67.76 366 72.33 0.879
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1122.6 3
0019-0072
 180.20 72.28 368 75.74 0.806
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 7028.2 3
0020-0072
 231.46 72.41 371 74.87 0.832
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 3623.6 3
0021-0073
 787.16 73.24 361 74.08 0.827
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 515.8 3
0022-0080
 733.46 80.24 325 113.64 0.316
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1153.2 3
0023-0086
 89.85 85.99 341 71.84 0.830
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4108.2 3
0024-0091
 165.10 92.46 712 105.40 0.805
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 269.4 3
0025-0088
 659.30 87.84 389 77.50 0.814
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1095.5 3
0026-0087
 685.00 87.49 373 74.33 0.848
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 6449.4 3
0027-0089
 473.46 89.44 376 74.91 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1197.2 3
0028-0091
 502.43 91.20 384 76.91 0.816
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1700.0 3
0029-0090
 753.67 90.95 380 74.08 0.870
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 327.4 3
0030-0093
 429.24 93.02 378 73.26 0.885
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2085.5 3
0031-0093
 230.83 92.37 369 72.18 0.890
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 681.6 3
0032-0097
 546.64 97.18 360 72.67 0.857
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 732.4 3
0033-0098
 833.39 98.43 356 70.18 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 217.8 3
0034-0105
 125.31 106.25 731 109.54 0.766
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 556.7 3
0035-0102
 376.54 102.10 373 73.84 0.860
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 368.7 3
0036-0106
 904.74 106.07 302 66.18 0.866
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 440.7 3
0037-0110
 488.71 110.65 412 86.57 0.691