Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2715.8 4
0124-0274
 894.80 274.10 305 65.60 0.891
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 220.2 4
0125-0278
 522.30 279.48 304 69.01 0.802
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1893.4 4
0126-0276
 602.65 276.21 294 62.77 0.938
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 626.0 4
0127-0284
 128.97 286.07 617 101.40 0.754
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 542.6 4
0128-0277
 254.08 276.78 243 59.36 0.867
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 500.6 4
0129-0286
 42.86 285.69 296 64.43 0.896
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1903.6 4
0130-0286
 158.65 286.05 218 55.70 0.883
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 368.0 4
0131-0288
 942.68 288.02 330 69.01 0.871
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 255.1 4
0132-0289
 406.39 289.20 301 67.01 0.842
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2236.6 4
0133-0289
 695.37 289.37 262 62.18 0.851
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 695.5 4
0134-0294
 340.79 295.23 513 90.91 0.780
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 825.6 4
0135-0293
 917.77 293.73 271 61.36 0.905
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 846.1 4
0136-0296
 250.70 296.90 422 77.84 0.875
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1744.5 4
0137-0293
 712.56 293.65 264 63.36 0.827
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 248.4 4
0138-0294
 560.23 293.96 274 64.43 0.830
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 297.9 4
0139-0299
 471.38 299.56 304 65.60 0.888
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 893.0 4
0140-0301
 392.38 301.52 280 64.18 0.854
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 628.4 4
0141-0307
 13.11 307.38 287 63.60 0.892
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 364.7 4
0142-0306
 80.29 306.31 181 50.87 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 467.4 4
0143-0307
 523.17 306.77 252 59.36 0.899
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 963.5 4
0144-0306
 612.94 307.01 227 54.77 0.951
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 255.3 4
0145-0309
 816.53 308.49 292 66.77 0.823
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 312.9 4
0146-0315
 425.75 315.11 295 63.01 0.934
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1901.7 4
0147-0319
 940.38 319.78 301 64.77 0.902
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 952.9 4
0148-0318
 803.67 318.62 290 69.84 0.747