Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 957.8 9
0054-0128
144.88 128.15 231 56.77 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 243.1 9
0055-0133
746.35 133.64 381 73.26 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 206.9 9
0056-0134
835.53 133.85 352 78.33 0.721
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 421.5 9
0057-0139
253.74 138.75 396 74.67 0.893
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1193.9 9
0058-0141
818.10 141.83 228 57.11 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 328.1 9
0059-0144
47.69 143.22 250 61.36 0.835
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 236.3 9
0060-0146
541.50 146.66 220 58.77 0.800
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 230.9 9
0061-0150
866.35 150.41 235 57.60 0.890
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1154.0 9
0062-0149
219.61 150.24 257 61.11 0.865
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 748.8 9
0063-0153
936.51 153.32 219 56.53 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 342.2 9
0064-0152
631.80 151.95 274 63.36 0.858
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1113.7 9
0065-0156
715.63 156.14 264 59.94 0.923
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1377.2 9
0066-0159
132.93 160.15 351 80.08 0.688
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1483.1 9
0067-0159
577.05 159.47 210 55.70 0.851
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1186.3 9
0068-0161
523.25 160.27 245 71.60 0.601
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 5604.0 9
0069-0166
589.08 166.62 279 68.18 0.754
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 381.8 9
0070-0168
857.63 168.79 337 69.01 0.889
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1625.9 9
0071-0168
163.28 168.37 218 58.77 0.793
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 249.3 9
0072-0167
753.10 167.95 345 77.36 0.725
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1162.4 9
0073-0170
813.41 169.51 207 59.11 0.744
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 231.4 9
0074-0173
337.38 173.65 437 79.84 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 205.6 9
0075-0173
53.07 173.78 231 57.94 0.865
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 197.7 9
0076-0177
659.41 177.28 409 77.01 0.867
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 370.4 9
0077-0177
903.32 177.17 420 77.26 0.884
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 864.1 9
0078-0177
119.07 177.60 157 47.70 0.867