Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 318.0 5
0348-0624
546.22 624.36 285 65.01 0.847
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 212.7 5
0349-0623
174.39 622.87 236 56.53 0.928
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 256.2 5
0350-0622
870.83 622.71 267 60.77 0.909
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 249.1 5
0351-0628
605.87 627.99 397 76.08 0.862
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 345.4 5
0352-0624
946.97 624.84 281 62.77 0.896
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 232.0 5
0353-0628
36.42 628.14 277 72.43 0.664
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 312.6 5
0354-0627
248.60 627.74 260 59.36 0.927
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2684.1 5
0355-0629
830.24 628.84 222 63.26 0.697
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 673.5 5
0356-0633
370.33 632.80 275 64.77 0.824
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 334.2 5
0357-0636
408.74 635.45 333 70.18 0.850
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1044.3 5
0358-0634
559.32 634.44 189 51.46 0.897
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1303.5 5
0359-0638
768.63 638.12 249 61.11 0.838
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 2039.9 5
0360-0644
477.73 644.58 285 65.60 0.832
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 269.6 5
0361-0646
141.04 646.00 238 58.53 0.873
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 393.9 5
0362-0645
624.71 644.66 226 55.70 0.915
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 782.6 5
0363-0648
565.94 648.44 244 60.53 0.837
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 318.1 5
0364-0655
923.94 655.47 386 75.60 0.849
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 221.8 5
0365-0654
227.52 653.58 294 63.60 0.913
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 599.8 5
0366-0653
685.29 652.45 228 58.77 0.830
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 191.4 5
0367-0655
855.20 655.65 287 62.18 0.933
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 583.4 5
0368-0656
278.26 655.64 249 62.53 0.800
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 655.8 5
0369-0655
514.61 654.75 228 56.53 0.897
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 264.0 5
0370-0656
649.18 656.57 234 57.94 0.876
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 498.8 5
0371-0656
726.71 655.54 244 57.94 0.913
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 625.3 5
0372-0663
819.55 663.14 272 61.94 0.891