Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2160.0 4
0301-0605
 933.74 605.18 223 55.94 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 184.8 4
0302-0606
 483.26 606.68 184 53.36 0.812
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 384.1 4
0303-0607
 792.06 607.40 263 59.94 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1066.7 4
0304-0610
 238.36 610.41 227 56.28 0.900
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 601.8 4
0305-0612
 213.73 611.44 215 55.70 0.871
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1777.2 4
0306-0615
 619.13 615.35 176 49.11 0.917
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 359.5 4
0307-0622
 857.02 622.48 252 59.36 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 628.5 4
0308-0620
 180.51 620.54 198 53.11 0.882
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 228.1 4
0309-0621
 647.58 621.74 256 60.18 0.888
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 200.9 4
0310-0626
 931.96 626.09 277 71.60 0.679
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 385.6 4
0311-0627
 369.29 627.77 272 62.18 0.884
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 843.9 4
0312-0628
 240.10 627.52 191 51.11 0.919
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 448.4 4
0313-0631
 112.43 631.22 295 62.77 0.941
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 1710.5 4
0314-0631
 594.04 631.05 250 59.36 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 256.2 4
0315-0634
 396.37 634.05 245 61.60 0.811
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 530.8 4
0316-0636
 439.91 636.09 375 71.60 0.919
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 247.4 4
0317-0638
 518.47 638.36 294 65.36 0.865
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 4916.1 4
0318-0638
 851.28 638.87 219 54.87 0.914
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2156.0 4
0319-0639
 71.19 639.60 175 49.80 0.887
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 277.8 4
0320-0643
 355.90 642.50 300 67.50 0.827
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 309.3 4
0321-0643
 720.20 643.02 246 59.60 0.870
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 249.0 4
0322-0650
 276.52 648.14 493 95.50 0.679
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 409.4 4
0323-0648
 471.83 647.96 344 69.84 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 2127.6 4
0324-0643
 623.44 642.89 206 61.36 0.688
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
 p21 202.7 4
0325-0651
 550.39 650.86 460 82.33 0.853