Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 608.7 7
0163-0336
 162.68 335.79 175 49.94 0.882
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.6 7
0164-0338
 896.94 338.98 324 67.84 0.885
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 551.1 7
0165-0338
 660.25 337.98 156 50.87 0.758
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 481.7 7
0166-0341
 646.64 340.59 261 61.11 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 484.3 7
0167-0340
 675.12 339.91 190 52.77 0.857
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 288.0 7
0168-0342
 707.87 342.91 276 69.84 0.711
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 729.8 7
0169-0342
 784.65 342.46 217 55.36 0.890
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 328.9 7
0170-0343
 309.89 343.09 258 60.18 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 587.9 7
0171-0346
 481.88 346.11 316 72.77 0.750
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 399.5 7
0172-0349
 217.80 349.32 361 71.01 0.900
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 417.9 7
0173-0347
 153.24 347.05 227 56.18 0.904
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 327.6 7
0174-0350
 859.26 351.10 336 68.53 0.899
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 557.0 7
0175-0349
 800.64 349.33 177 49.70 0.901
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 255.7 7
0176-0353
 338.93 352.34 261 60.77 0.888
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 419.1 7
0177-0358
 71.12 358.95 447 91.74 0.667
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 419.6 7
0178-0353
 817.46 353.46 233 59.70 0.822
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 538.5 7
0179-0362
 371.36 361.56 494 93.05 0.717
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 421.6 7
0180-0367
 99.04 367.18 454 85.74 0.776
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 669.3 7
0181-0359
 462.03 360.16 146 45.21 0.897
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 277.7 7
0182-0369
 691.26 369.39 264 64.08 0.808
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 377.5 7
0183-0370
 447.16 369.83 216 55.11 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 355.7 7
0184-0370
 843.94 370.61 319 66.53 0.906
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 216.0 7
0185-0371
 278.60 372.16 262 63.11 0.827
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 294.6 7
0186-0375
 503.14 375.57 260 65.36 0.765
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 275.0 7
0187-0374
 307.18 373.51 277 65.36 0.815