Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 247.6 3
0263-0442
807.62 442.76 329 75.26 0.730
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 194.6 3
0264-0443
888.83 442.33 332 69.26 0.870
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 488.0 3
0265-0443
531.39 443.83 328 67.01 0.918
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 263.0 3
0266-0450
31.80 450.55 432 79.01 0.870
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 430.0 3
0267-0449
933.81 450.02 256 61.11 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1987.9 3
0268-0451
72.30 451.27 263 62.18 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 8607.4 3
0269-0453
371.01 453.85 319 68.67 0.850
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 288.5 3
0270-0451
699.44 451.53 254 59.36 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 188.3 3
0271-0455
185.28 455.49 283 65.01 0.841
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 704.3 3
0272-0453
247.81 453.68 216 55.11 0.894
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 196.4 3
0273-0455
748.33 454.75 174 50.63 0.853
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 163.2 3
0274-0454
759.92 453.92 166 49.80 0.841
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3287.7 3
0275-0454
508.66 454.74 219 59.36 0.781
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 1348.1 3
0276-0459
681.14 458.94 406 74.67 0.915
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 4345.0 3
0277-0458
140.86 458.00 306 64.77 0.917
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 317.2 3
0278-0458
447.06 457.61 270 62.18 0.877
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3764.6 3
0279-0458
311.78 458.48 236 58.18 0.876
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 300.1 3
0280-0461
92.82 461.21 289 63.36 0.905
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 470.0 3
0281-0466
276.12 465.89 268 62.77 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 802.5 3
0282-0466
422.12 466.59 247 57.94 0.925
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 3797.6 3
0283-0469
324.75 469.28 258 60.77 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 359.8 3
0284-0469
295.54 469.85 274 61.94 0.897
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 250.3 3
0285-0473
383.72 473.38 260 61.01 0.878
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 492.0 3
0286-0472
836.60 472.36 236 57.11 0.909
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
p21 / Alexa Fluor 647 rabbit IgG(H+L) / goat / cross-adsorbed
Cy5
p21 244.5 3
0287-0476
177.39 476.77 357 72.33 0.858