Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 411.5 7
0053-0663
760.22 663.21 252 61.60 0.835
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 390.9 7
0054-0665
58.61 665.53 298 63.94 0.916
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 398.1 7
0055-0677
779.19 677.03 238 61.60 0.788
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 638.7 7
0056-0692
383.49 691.93 179 49.70 0.911
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 453.2 7
0057-0691
471.18 691.53 195 56.87 0.758
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 275.5 8
0001-0031
936.98 31.62 278 65.60 0.812
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 441.5 8
0002-0054
801.15 53.88 209 56.87 0.812
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 412.7 8
0003-0061
843.75 61.43 216 66.04 0.622
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 457.9 8
0004-0072
744.01 72.77 201 59.70 0.709
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 956.3 8
0005-0110
290.92 109.83 264 63.01 0.836
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 595.2 8
0006-0124
868.05 124.44 204 55.36 0.837
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 470.7 8
0007-0136
397.97 136.22 279 62.53 0.897
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 633.5 8
0008-0157
297.33 157.19 199 51.11 0.957
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 389.0 8
0009-0177
236.55 176.67 260 64.53 0.785
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 659.2 8
0010-0177
353.95 177.55 204 52.28 0.938
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 423.7 8
0011-0182
141.63 182.29 215 60.43 0.740
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 507.5 8
0012-0184
417.53 184.50 266 63.84 0.820
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 669.7 8
0013-0195
580.65 195.20 189 53.11 0.842
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 441.6 8
0014-0207
918.96 206.97 220 55.60 0.894
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 441.7 8
0015-0214
236.87 214.53 211 59.01 0.761
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 497.2 8
0016-0241
897.47 241.14 199 55.36 0.816
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 491.5 8
0017-0260
527.98 260.45 217 56.18 0.864
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 489.5 8
0018-0264
450.21 263.65 272 63.36 0.852
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 378.3 8
0019-0297
861.92 297.03 274 63.94 0.842
COLO 858 Vemurafenib
3.16
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 373.6 8
0020-0298
714.96 298.45 291 64.18 0.888