Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1958.7 5
0031-0080
 123.83 80.49 366 76.18 0.792
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 332.9 5
0032-0075
 165.25 74.41 288 65.70 0.838
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 346.4 5
0033-0078
 613.99 77.76 256 58.53 0.939
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 282.3 5
0034-0079
 923.97 79.38 259 59.70 0.913
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 346.6 5
0035-0081
 60.39 81.99 265 64.18 0.808
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 342.3 5
0036-0084
 505.04 84.26 310 65.94 0.896
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 274.1 5
0037-0090
 693.12 90.15 316 68.18 0.854
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 467.3 5
0038-0088
 724.22 87.61 299 63.60 0.929
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 475.0 5
0039-0087
 254.08 87.36 238 58.77 0.866
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 283.3 5
0040-0089
 87.63 89.57 252 58.53 0.924
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1728.3 5
0041-0090
 184.69 90.96 419 81.26 0.797
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2881.5 5
0042-0094
 281.23 94.20 346 70.18 0.883
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 350.2 5
0043-0093
 162.46 93.63 217 59.70 0.765
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 288.3 5
0044-0097
 316.17 98.03 272 60.77 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 379.2 5
0045-0105
 858.99 106.45 309 76.77 0.659
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 333.4 5
0046-0101
 651.89 101.10 345 69.84 0.889
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 279.1 5
0047-0102
 503.46 101.78 273 62.77 0.871
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 343.0 5
0048-0105
 894.66 104.84 302 69.84 0.778
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 312.1 5
0049-0105
 342.24 105.07 235 55.94 0.944
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 295.8 5
0050-0107
 788.00 107.40 286 64.77 0.857
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 284.4 5
0051-0109
 47.58 108.98 289 62.77 0.922
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 3986.1 5
0052-0108
 208.12 107.97 321 66.18 0.921
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 504.8 5
0053-0109
 829.80 108.41 263 60.77 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 477.7 5
0054-0110
 550.61 109.80 235 57.46 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1992.1 5
0055-0114
 939.47 113.65 286 65.94 0.827