Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 295.3 1
0290-0550
835.32 549.74 270 61.36 0.901
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 441.2 1
0291-0549
145.49 549.09 265 62.04 0.865
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 394.8 1
0292-0552
920.15 551.59 294 63.36 0.920
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 400.9 1
0293-0553
312.34 553.07 308 65.01 0.916
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3109.1 1
0294-0553
786.10 553.31 341 71.84 0.830
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 892.9 1
0295-0556
560.59 557.44 266 59.60 0.941
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 589.5 1
0296-0559
390.52 558.51 297 64.77 0.890
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 568.0 1
0297-0563
482.05 562.86 252 58.18 0.935
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 501.8 1
0298-0563
675.45 563.22 272 63.11 0.858
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 293.1 1
0299-0564
92.34 564.16 140 51.11 0.673
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2008.2 1
0300-0569
793.86 569.65 318 66.77 0.896
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1121.3 1
0301-0567
268.09 568.13 164 47.21 0.925
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 416.1 1
0302-0571
593.86 571.40 272 60.53 0.933
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 300.4 1
0303-0572
205.22 572.62 276 67.60 0.759
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3690.0 1
0304-0571
518.31 570.42 129 68.53 0.345
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2921.9 1
0305-0579
898.28 579.25 364 71.60 0.892
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 445.9 1
0306-0575
245.48 575.14 169 47.46 0.943
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 505.7 1
0307-0576
559.64 575.69 245 58.53 0.899
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1549.5 1
0308-0578
732.89 578.70 294 64.77 0.881
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 398.8 1
0309-0580
875.88 579.73 285 62.18 0.926
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 313.1 1
0310-0581
82.77 581.36 293 63.36 0.917
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 848.6 1
0311-0587
262.45 585.43 422 83.60 0.759
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 749.2 1
0312-0584
449.27 583.89 246 57.11 0.948
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1136.6 1
0313-0585
537.45 585.66 219 55.36 0.898
COLO 858 Vemurafenib
0.0316
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 368.2 1
0314-0588
301.52 587.94 322 67.60 0.886