Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 697.3 8
0224-0367
825.59 367.27 264 63.01 0.836
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1819.7 8
0225-0368
69.91 368.36 423 77.60 0.883
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 421.1 8
0226-0367
509.82 366.75 165 49.11 0.860
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 329.8 8
0227-0370
842.54 368.99 221 57.36 0.844
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2504.4 8
0228-0370
904.68 370.38 299 63.94 0.919
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2272.5 8
0229-0374
705.18 375.56 291 65.36 0.856
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2221.1 8
0230-0380
229.26 379.63 336 69.01 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1968.1 8
0231-0381
625.58 381.14 261 59.94 0.913
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2865.3 8
0232-0380
538.41 379.26 186 52.28 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2118.9 8
0233-0380
122.53 380.11 176 49.46 0.904
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2644.0 8
0234-0390
459.42 389.07 472 94.81 0.660
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1635.9 8
0235-0385
174.02 384.99 229 55.11 0.947
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2526.5 8
0236-0390
32.31 390.91 315 65.36 0.927
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1573.4 8
0237-0390
476.96 389.92 250 59.46 0.889
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3006.3 8
0238-0389
563.53 389.04 227 56.04 0.908
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 300.0 8
0239-0392
96.37 392.44 221 54.53 0.934
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1801.8 8
0240-0392
140.30 393.04 241 60.53 0.827
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3065.1 8
0241-0394
517.34 394.43 226 57.36 0.863
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 377.6 8
0242-0393
205.01 393.33 193 53.11 0.860
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2553.4 8
0243-0396
357.48 395.76 326 68.43 0.875
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1172.8 8
0244-0396
798.26 395.72 268 59.60 0.948
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1903.9 8
0245-0393
115.88 393.18 177 49.70 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1440.0 8
0246-0401
285.34 401.61 298 68.67 0.794
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1045.9 8
0247-0403
854.48 404.25 271 62.53 0.871
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3614.6 8
0248-0409
874.03 409.15 215 59.36 0.767