Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1239.4 7
0379-0706
646.96 706.78 211 53.11 0.940
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1008.2 7
0380-0708
161.23 708.82 276 64.53 0.833
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 587.5 8
0001-0027
605.94 27.87 215 56.28 0.853
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1609.1 8
0002-0030
678.63 29.96 293 63.01 0.927
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 418.8 8
0003-0030
332.84 29.76 212 53.94 0.916
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1403.5 8
0004-0034
100.59 34.63 237 58.53 0.869
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 307.8 8
0005-0030
274.05 30.58 219 59.70 0.772
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1511.9 8
0006-0039
437.09 39.00 204 53.36 0.901
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 459.2 8
0007-0041
321.69 41.81 212 54.28 0.904
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3114.4 8
0008-0040
928.24 40.30 223 54.28 0.951
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1166.9 8
0009-0043
461.70 44.14 285 63.60 0.885
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1782.5 8
0010-0044
809.55 44.14 285 61.36 0.951
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 544.5 8
0011-0045
569.92 43.93 174 54.43 0.738
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 427.3 8
0012-0045
358.74 45.50 219 55.11 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2679.1 8
0013-0045
633.25 45.14 254 60.18 0.881
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 371.5 8
0014-0047
122.29 46.71 280 66.18 0.803
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1787.5 8
0015-0048
25.24 47.86 211 53.11 0.940
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 4661.7 8
0016-0048
185.64 48.59 289 64.18 0.882
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2250.8 8
0017-0049
384.18 48.78 310 66.53 0.880
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 375.7 8
0018-0049
307.42 48.87 215 54.87 0.897
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 441.7 8
0019-0050
580.11 51.04 112 44.87 0.699
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 435.3 8
0020-0053
753.84 52.66 215 54.28 0.917
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3986.0 8
0021-0053
254.99 53.81 251 60.53 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1902.9 8
0022-0056
493.18 56.48 338 69.01 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1726.7 8
0023-0054
940.44 54.25 205 52.28 0.942