Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 692.8 2
0239-0481
811.78 481.81 243 57.36 0.928
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1815.1 2
0240-0482
346.62 482.36 157 46.87 0.898
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1768.9 2
0241-0485
334.30 485.46 209 58.04 0.780
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 732.1 2
0242-0487
401.77 486.60 330 85.98 0.561
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2890.6 2
0243-0484
794.52 484.85 134 44.04 0.868
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1618.2 2
0244-0490
268.59 489.77 219 55.70 0.887
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1643.5 2
0245-0495
739.34 495.18 514 90.91 0.782
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 394.2 2
0246-0489
873.21 488.82 152 47.70 0.840
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2402.9 2
0247-0496
912.94 496.14 442 88.57 0.708
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1328.4 2
0248-0498
569.45 497.41 356 72.77 0.845
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2231.4 2
0249-0496
153.46 496.54 305 63.36 0.955
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3064.2 2
0250-0496
181.71 495.69 317 65.60 0.926
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1922.2 2
0251-0495
689.16 495.01 254 63.36 0.795
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 400.0 2
0252-0495
883.61 495.94 150 46.28 0.880
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1861.8 2
0253-0508
654.92 508.22 408 73.94 0.938
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2828.3 2
0254-0509
417.01 509.34 242 57.94 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2178.3 2
0255-0512
221.16 512.13 295 65.60 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2147.9 2
0256-0512
503.00 512.88 347 68.18 0.938
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 418.7 2
0257-0517
108.91 517.87 304 71.26 0.752
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2203.6 2
0258-0518
598.24 518.38 446 79.60 0.885
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 2323.8 2
0259-0519
266.95 518.57 261 62.18 0.848
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1229.6 2
0260-0516
810.10 516.24 257 60.53 0.882
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1271.7 2
0261-0516
901.90 515.64 216 56.53 0.849
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 1878.4 2
0262-0520
54.58 519.61 347 69.26 0.909
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-2
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
Rb (pS807; pS811) 3155.5 2
0263-0517
693.80 518.29 260 60.28 0.899