Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.8 3
0023-0177
832.00 178.04 298 65.26 0.879
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 232.8 3
0024-0180
761.07 179.65 319 70.18 0.814
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 378.2 3
0025-0177
491.67 177.39 215 55.11 0.889
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 223.2 3
0026-0188
796.60 188.30 334 69.36 0.873
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 778.7 3
0027-0192
111.65 192.79 198 51.11 0.952
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 355.1 3
0028-0201
382.46 201.75 271 63.26 0.851
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 277.8 3
0029-0205
201.11 204.61 346 71.11 0.860
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 281.7 3
0030-0209
706.89 208.43 356 71.84 0.867
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 319.8 3
0031-0212
450.10 211.48 257 58.77 0.935
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.9 3
0032-0220
833.52 220.04 327 70.67 0.823
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 423.2 3
0033-0220
134.00 220.04 250 59.60 0.884
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.9 3
0034-0227
404.98 227.52 305 64.77 0.914
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 349.2 3
0035-0230
513.44 230.31 226 62.77 0.721
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 494.5 3
0036-0234
547.93 234.56 240 58.53 0.880
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 316.3 3
0037-0237
281.30 237.36 268 63.11 0.845
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 379.8 3
0038-0255
675.34 254.68 236 58.53 0.866
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 281.3 3
0039-0258
799.14 258.45 311 66.43 0.886
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 480.8 3
0040-0262
553.47 262.69 241 58.53 0.884
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 279.2 3
0041-0266
25.16 265.73 251 63.01 0.794
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.6 3
0042-0265
863.12 265.41 274 63.60 0.851
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 357.7 3
0043-0265
141.87 264.90 244 59.94 0.853
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 491.5 3
0044-0269
487.64 268.24 132 54.77 0.553
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 320.1 3
0045-0276
280.84 276.59 386 73.26 0.904
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 359.4 3
0046-0283
412.01 282.93 270 61.36 0.901
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 441.3 3
0047-0287
480.67 286.13 285 76.33 0.615