Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 261.7 2
0078-0397
 212.63 398.04 322 65.60 0.940
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 268.5 2
0079-0404
 652.55 404.88 359 70.77 0.901
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 249.7 2
0080-0409
 431.96 408.57 379 72.67 0.902
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.6 2
0081-0430
 485.77 430.66 314 68.18 0.849
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 253.7 2
0082-0436
 193.76 436.75 316 68.43 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 342.1 2
0083-0436
 587.81 435.82 323 69.01 0.852
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 337.6 2
0084-0437
 42.06 436.96 271 61.11 0.912
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 869.3 2
0085-0437
 821.66 437.77 223 56.77 0.870
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 583.9 2
0086-0448
 660.63 448.83 239 58.53 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 223.5 2
0087-0453
 54.39 453.00 355 70.77 0.891
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 350.7 2
0088-0459
 340.67 458.85 249 61.84 0.818
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 452.1 2
0089-0458
 740.67 458.64 236 59.70 0.832
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 235.3 2
0090-0467
 519.60 467.21 345 69.36 0.901
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 425.1 2
0091-0479
 573.14 478.52 194 66.43 0.552
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 300.2 2
0092-0489
 169.47 489.35 315 70.77 0.790
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 266.4 2
0093-0519
 590.31 518.90 428 82.08 0.798
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 292.5 2
0094-0517
 202.43 517.49 317 67.36 0.878
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 368.7 2
0095-0523
 87.21 522.84 469 81.50 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 398.3 2
0096-0521
 771.81 521.83 313 68.77 0.832
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 291.4 2
0097-0538
 125.28 538.27 288 62.53 0.926
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 301.9 2
0098-0537
 646.98 536.44 248 62.18 0.806
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.4 2
0099-0545
 186.16 545.47 349 70.43 0.884
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 309.7 2
0100-0562
 460.22 561.46 366 74.43 0.830
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.3 2
0101-0559
 846.47 559.65 280 61.60 0.927
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 286.0 2
0102-0581
 830.65 580.36 296 64.77 0.887