Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 325.3 7
0107-0342
176.49 342.38 406 75.26 0.901
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.9 7
0108-0352
838.85 352.49 419 77.84 0.869
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 243.3 7
0109-0353
37.15 353.91 411 77.26 0.865
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 408.9 7
0110-0354
196.64 354.38 249 64.18 0.760
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.8 7
0111-0357
911.74 356.92 410 77.50 0.858
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.4 7
0112-0362
721.35 361.82 401 73.60 0.930
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 308.6 7
0113-0375
307.67 373.54 822 118.37 0.737
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 245.8 7
0114-0372
576.22 372.32 426 78.08 0.878
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 285.1 7
0115-0379
255.42 379.49 555 88.33 0.894
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 268.8 7
0116-0379
104.36 379.35 412 75.84 0.900
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 249.7 7
0117-0383
353.55 383.66 403 78.08 0.831
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 213.8 7
0118-0393
729.49 393.01 419 76.43 0.901
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 283.7 7
0119-0399
411.40 399.13 422 78.67 0.857
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 231.1 7
0120-0400
896.04 400.17 430 77.01 0.911
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.7 7
0121-0412
547.67 412.36 432 77.26 0.910
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 276.8 7
0122-0417
477.90 416.96 424 78.43 0.866
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 240.6 7
0123-0421
798.64 421.39 816 108.13 0.877
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.2 7
0124-0418
288.39 418.77 420 76.67 0.898
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 284.3 7
0125-0422
221.78 422.33 394 75.84 0.861
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 211.8 7
0126-0427
674.72 427.78 474 84.57 0.833
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 254.7 7
0127-0435
647.25 434.77 971 126.37 0.764
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 268.2 7
0128-0432
22.64 432.45 395 74.67 0.890
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.2 7
0129-0428
852.61 427.21 311 66.77 0.877
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 299.2 7
0130-0436
442.88 436.49 421 78.67 0.855
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 327.4 7
0131-0438
413.29 438.11 427 78.91 0.862