Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 315.5 1
0025-0121
 127.38 120.85 508 89.74 0.793
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.6 1
0026-0128
 900.25 128.11 430 79.60 0.853
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 310.6 1
0027-0133
 881.34 132.98 363 74.18 0.829
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 334.2 1
0028-0136
 456.29 136.38 497 86.77 0.830
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 348.0 1
0029-0143
 481.89 143.29 489 88.67 0.782
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 472.2 1
0030-0146
 52.86 146.04 428 76.43 0.921
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 321.6 1
0031-0149
 733.33 148.93 470 84.08 0.835
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 245.4 1
0032-0152
 636.15 151.24 411 81.50 0.778
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.5 1
0033-0156
 179.65 155.98 413 75.84 0.902
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 315.1 1
0034-0158
 695.52 158.70 454 79.26 0.908
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 323.1 1
0035-0163
 356.63 163.51 438 80.67 0.846
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 567.2 1
0036-0167
 538.52 166.59 422 77.26 0.889
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.5 1
0037-0167
 625.67 168.24 495 85.74 0.846
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 524.4 1
0038-0181
 256.69 180.51 798 105.40 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 401.3 1
0039-0177
 940.24 176.77 643 113.98 0.622
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 378.4 1
0040-0182
 919.56 184.38 246 70.43 0.623
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 290.4 1
0041-0188
 417.75 187.91 483 82.91 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 278.7 1
0042-0193
 890.39 193.06 442 76.67 0.945
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 285.1 1
0043-0198
 114.34 198.34 433 77.84 0.898
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.8 1
0044-0197
 159.98 196.69 427 75.84 0.933
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 331.3 1
0045-0199
 194.67 198.65 454 80.67 0.877
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 324.7 1
0046-0201
 473.12 201.79 429 77.26 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 276.3 1
0047-0203
 52.74 203.81 405 75.84 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 258.5 1
0048-0205
 90.17 204.94 458 80.91 0.879
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.0 1
0049-0205
 597.63 204.93 433 76.43 0.932