Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 237.0 8
0110-0342
 786.67 342.22 406 76.67 0.868
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 266.4 8
0111-0348
 449.67 347.65 724 104.91 0.827
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.2 8
0112-0347
 166.15 347.14 412 77.50 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 260.9 8
0113-0358
 547.03 358.86 868 122.85 0.723
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 319.6 8
0114-0349
 211.63 349.20 437 79.50 0.869
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.7 8
0115-0357
 711.60 357.80 419 78.91 0.846
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 215.3 8
0116-0363
 399.47 362.29 445 81.84 0.835
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.6 8
0117-0372
 497.55 372.35 460 81.84 0.863
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 225.1 8
0118-0374
 757.54 374.67 449 80.91 0.862
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.0 8
0119-0376
 601.74 376.51 426 79.74 0.842
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 210.9 8
0120-0378
 784.90 377.44 438 79.50 0.871
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 215.8 8
0121-0381
 304.43 382.27 420 80.18 0.821
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 206.6 8
0122-0389
 60.15 389.85 389 76.33 0.839
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 261.2 8
0123-0393
 428.91 394.39 864 118.37 0.775
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 287.5 8
0124-0400
 117.36 399.99 841 114.13 0.811
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.0 8
0125-0391
 520.05 391.20 456 84.91 0.795
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.9 8
0126-0394
 689.93 393.77 429 76.43 0.923
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 270.9 8
0127-0396
 907.28 396.73 421 79.84 0.830
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 208.6 8
0128-0401
 177.10 400.90 434 80.08 0.850
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 187.7 8
0129-0403
 15.32 402.92 373 72.43 0.894
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 225.4 8
0130-0407
 308.11 406.08 476 84.77 0.832
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.5 8
0131-0405
 651.10 405.55 421 78.08 0.868
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 324.3 8
0132-0411
 850.74 411.27 371 71.60 0.909
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.6 8
0133-0413
 581.10 413.40 417 79.74 0.824
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 324.7 8
0134-0426
 901.74 426.81 747 111.15 0.760