Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 278.7 7
0121-0422
462.95 422.00 463 84.57 0.814
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 276.5 7
0122-0428
737.20 428.53 799 106.23 0.890
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.7 7
0123-0426
357.00 426.46 420 78.91 0.848
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.3 7
0124-0433
207.82 432.94 401 76.67 0.857
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 245.3 7
0125-0439
548.38 439.23 853 114.71 0.815
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.1 7
0126-0445
130.87 445.13 436 81.15 0.832
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 214.7 7
0127-0446
872.69 446.15 435 79.50 0.865
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.2 7
0128-0450
177.60 449.89 471 86.57 0.790
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 438.3 7
0129-0458
504.55 457.94 831 112.13 0.831
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.2 7
0130-0453
617.91 452.19 430 79.98 0.845
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 263.0 7
0131-0460
330.60 460.23 433 78.08 0.892
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.3 7
0132-0469
633.26 470.21 488 85.40 0.841
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.2 7
0133-0468
685.96 468.34 437 78.91 0.882
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.7 7
0134-0469
439.37 469.24 443 79.84 0.873
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 377.9 7
0135-0469
568.45 470.06 280 84.08 0.498
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 246.9 7
0136-0482
928.02 481.95 894 116.95 0.821
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 235.5 7
0137-0483
224.25 482.98 421 78.91 0.850
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.8 7
0138-0488
77.97 488.45 444 86.23 0.750
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 251.1 7
0139-0489
119.46 489.20 400 77.74 0.832
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 278.7 7
0140-0503
605.96 503.79 865 116.95 0.795
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 263.6 7
0141-0497
448.54 497.80 458 85.15 0.794
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.4 7
0142-0497
732.17 496.51 436 82.33 0.808
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.1 7
0143-0497
846.56 496.51 448 80.67 0.865
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 282.9 7
0144-0503
791.27 504.05 818 111.40 0.828
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 262.1 7
0145-0504
534.13 504.29 423 77.26 0.891