Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 193.1 4
0180-0594
470.06 594.13 422 77.01 0.894
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 220.6 4
0181-0595
357.99 594.48 437 77.84 0.906
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 453.0 4
0182-0601
707.62 600.99 472 81.84 0.886
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.9 4
0183-0603
861.60 603.94 748 106.91 0.822
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 238.6 4
0184-0602
593.70 602.36 432 78.67 0.877
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 321.9 4
0185-0605
803.17 605.81 480 84.53 0.844
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 262.4 4
0186-0605
189.40 604.80 467 80.67 0.902
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 230.1 4
0187-0605
682.74 605.51 455 78.67 0.924
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 255.2 4
0188-0608
829.34 608.71 803 110.53 0.826
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 217.8 4
0189-0609
407.77 609.26 430 77.84 0.892
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 244.3 4
0190-0617
48.28 617.11 419 76.08 0.910
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 306.1 4
0191-0618
73.94 618.25 435 80.33 0.847
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 316.7 4
0192-0620
534.59 620.39 454 83.15 0.825
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 306.7 4
0193-0629
652.35 629.74 426 76.43 0.917
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 204.7 4
0194-0630
464.45 630.65 459 81.50 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.9 4
0195-0631
347.40 631.22 432 76.43 0.929
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 349.6 4
0196-0637
166.84 637.00 673 98.81 0.866
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 300.5 4
0197-0633
766.19 633.25 448 77.84 0.929
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 334.0 4
0198-0637
824.14 634.50 444 83.50 0.800
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 213.7 4
0199-0647
616.31 647.51 434 80.08 0.850
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 181.6 4
0200-0648
893.26 647.73 452 83.74 0.810
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.8 4
0201-0647
413.25 647.58 436 79.74 0.862
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 273.6 4
0202-0649
811.68 650.31 462 82.47 0.854
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 307.8 4
0203-0655
284.65 655.64 972 129.68 0.726
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 242.6 4
0204-0650
16.51 650.72 409 75.84 0.894